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SPE optimization for peptide sample preparation for urine peptidomics

ABSTRACT: Development of a modified solid phase extraction (SPE) approach for improved peptide yield and analysis sensitivity with LC-MS based peptidomics of urine.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Dr Minnie M. Sarwal

PROVIDER: MSV000080086 | MassIVE | Tue Aug 23 00:21:00 BST 2016

SECONDARY ACCESSION(S): PXD003045

REPOSITORIES: MassIVE

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Optimization for peptide sample preparation for urine peptidomics.

Sigdel Tara K TK Nicora Carrie D CD Hsieh Szu-Chuan SC Dai Hong H Qian Wei-Jun WJ Camp David G DG Sarwal Minnie M MM

Clinical proteomics 20140225 1

Analysis of native or endogenous peptides in biofluids can provide valuable insights into disease mechanisms. Furthermore, the detected peptides may also have utility as potential biomarkers for non-invasive monitoring of human diseases. The non-invasive nature of urine collection and the abundance of peptides in the urine makes analysis by high-throughput 'peptidomics' methods , an attractive approach for investigating the pathogenesis of renal disease. However, urine peptidomics methodologies ...[more]

PMID: 24568099

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