Proteomic analysis of integrin-associated adhesion complexes isolated from cells treated with DMSO or FAK inhibitor

ABSTRACT: Human foreskin fibroblast cells were spread on fibronectin (FN) for 1 h and treated with DMSO or FAK inhibitor (3 uM, AZ13256675 (termed AZ675 for short)) for 1 h. Integrin-associated adhesion complexes isolated from these cells were analysed by mass spectrometry to determine changes in adhesion complex composition upon FAK inhibition. As a negative control, complexes were also isolated from cells attached to transferrin (Tf), which allows integrin-independent adhesion via the transferrin receptor.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Dr Martin J. Humphries 

PROVIDER: MSV000080089 | MassIVE | Tue Aug 23 00:31:00 BST 2016

SECONDARY ACCESSION(S): PXD002720

REPOSITORIES: MassIVE