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DiDO-IPTL method publication data

ABSTRACT: Data demonstrating utility of diDO-IPTL labeling method for quantitative proteomics

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Desulfovibrio Vulgaris Str. Hildenborough (ncbitaxon:882)

SUBMITTER: Jacob Waldbauer

PROVIDER: MSV000080457 | MassIVE | Sun Jan 08 13:05:00 GMT 2017

REPOSITORIES: MassIVE

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diDO-IPTL: A Peptide-Labeling Strategy for Precision Quantitative Proteomics.

Waldbauer Jacob J Zhang Lichun L Rizzo Adriana A Muratore Daniel D

Analytical chemistry 20171023 21

We present an analytical strategy, dimethylation-deuteration and oxygen-exchange IPTL (diDO-IPTL), for high-precision, broad-coverage quantitative proteomics. The diDO-IPTL approach combines two advances in isobaric peptide terminal labeling (IPTL) methodology: first, a one-pot chemical labeling strategy for attaching isotopic tags to both the N- and C-termini of tryptic peptides, and second, a search engine (based on the Morpheus algorithm) optimized for identification and quantification of twi ...[more]

PMID: 29016104

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