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Human HeLa cell lince SILAC

ABSTRACT: The arginylation branch of the N-end rule pathway positively regulates cellular autophagic flux and clearance of proteotoxic protein

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Min Jae Lee

PROVIDER: MSV000080791 | MassIVE | Wed Mar 29 15:25:00 BST 2017

SECONDARY ACCESSION(S): PXD003813

REPOSITORIES: MassIVE

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The arginylation branch of the N-end rule pathway positively regulates cellular autophagic flux and clearance of proteotoxic proteins.

Jiang Yanxialei Y Lee Jeeyoung J Lee Jung Hoon JH Lee Joon Won JW Kim Ji Hyeon JH Choi Won Hoon WH Yoo Young Dong YD Cha-Molstad Hyunjoo H Kim Bo Yeon BY Kwon Yong Tae YT Noh Sue Ah SA Kim Kwang Pyo KP Lee Min Jae MJ

Autophagy 20160825 11

The N-terminal amino acid of a protein is an essential determinant of ubiquitination and subsequent proteasomal degradation in the N-end rule pathway. Using para-chloroamphetamine (PCA), a specific inhibitor of the arginylation branch of the pathway (Arg/N-end rule pathway), we identified that blocking the Arg/N-end rule pathway significantly impaired the fusion of autophagosomes with lysosomes. Under ER stress, ATE1-encoded Arg-tRNA-protein transferases carry out the N-terminal arginylation of ...[more]

PMID: 27560450

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Human HeLa cell lince SILAC - The arginylation branch of the N-end rule pathway positively regulates cellular autophagic flux and clearance of proteotoxic proteins

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