MudPIT analyses of the human proteins biotinylated by proximity to exogenously expressed biotin ligase (BioID)

ABSTRACT: To identify non-specific biotinylation events, HEK293 cells transfected with a plasmid carrying only the biotin ligase moiety were used as negative controls to BioID experiments. Cells were lysed in the presence of detergents. After purification of biotinylated proteins on streptavidin-conjugated beads, the eluates were precipitated with TCA. After washing with acetone, the protein mixtures were digested with endoproteinase Lys-C and trypsin (Promega) and analyzed by MudPIT.

INSTRUMENT(S): LTQ

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Laurence Florens  

PROVIDER: MSV000080809 | MassIVE | Thu Mar 30 11:44:00 BST 2017

SECONDARY ACCESSION(S): PXD006222

REPOSITORIES: MassIVE