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Cerebrospinal fluid markers to distinguish bacterial meningitis from cerebral malaria in children

ABSTRACT: Clinically distinguishing acute bacterial meningitis from cerebral malaria, both of which are important causes of acute non-traumatic coma associated with morbidity and mortality among paediatric hospital admissions in malaria endemic areas of Africa, is challenging. Few hospitals have diagnostic capacity for distinguishing the two syndromes resulting in broader antibiotic cover than necessary. A biochemical marker of ABM would facilitate precise clinical diagnosis and management of these infections. In this study, we used Label-free protein quantification by mass spectrometry to identify components of the CSF protein expression profiles of children with ABM that distinguish from those with CM with a high degree of specificity and sensitivity and that could be developed into point-of-care tests. James M. Njunge, Ian N. Oyaro, Nelson K. Kibinge, Martin K. Rono, Symon Kariuki, Charles R. Newton, James A. Berkley, Evelyn Gitau

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: James M. Njunge

PROVIDER: MSV000080979 | MassIVE | Mon Apr 24 01:23:00 BST 2017

SECONDARY ACCESSION(S): PXD006357

REPOSITORIES: MassIVE

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Cerebrospinal fluid markers to distinguish bacterial meningitis from cerebral malaria in children.

Njunge James M JM Oyaro Ian N IN Kibinge Nelson K NK Rono Martin K MK Kariuki Symon M SM Newton Charles R CR Berkley James A JA Gitau Evelyn N EN

Wellcome open research 20170926

Background. Few hospitals in high malaria endemic countries in Africa have the diagnostic capacity for clinically distinguishing acute bacterial meningitis (ABM) from cerebral malaria (CM). As a result, empirical use of antibiotics is necessary. A biochemical marker of ABM would facilitate precise clinical diagnosis and management of these infections and enable rational use of antibiotics. Methods. We used label-free protein quantification by mass spectrometry to identify ce ...[more]

PMID: 29181450

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