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Yeast Multiplexed HyCCAPP

ABSTRACT: Bottom-up proteomics data from a study of proteins associated with yeast's ribosome biogenesis and stress response genes (four genomic loci: 25S rDNA, ARX1, CTT1 and RPL30), using multiplexed HyCCAPP (Hybridization Capture of Chromatin-Associated Protein for Proteomics). Data include spectra from 4 loci capture samples, 1 uncaptured lysate control, with 2 technical replicates for each of the 3 biological replicates.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Saccharomyces Cerevisiae (ncbitaxon:4932)

SUBMITTER: Lloyd M. Smith

PROVIDER: MSV000081066 | MassIVE | Mon May 08 15:41:00 BST 2017

REPOSITORIES: MassIVE

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Publications

Multiplexed Sequence-Specific Capture of Chromatin and Mass Spectrometric Discovery of Associated Proteins.

Dai Yunxiang Y Kennedy-Darling Julia J Shortreed Michael R MR Scalf Mark M Gasch Audrey P AP Smith Lloyd M LM

Analytical chemistry 20170711 15

Comprehensive understanding of a gene's expression and regulation at the molecular level requires identification of all proteins interacting with the gene. HyCCAPP (Hybridization Capture of Chromatin Associated Proteins for Proteomics) is an approach that uses single-stranded DNA oligonucleotides to capture specific genomic sequences in cross-linked chromatin fragments and identify associated proteins by mass spectrometry. Previous studies have shown HyCCAPP to provide useful information on prot ...[more]

PMID: 28654248

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