Spectral library of Drosophila melanogaster for SWATH-MS

ABSTRACT: A spectral library was built for Drosophila melanogaster. The spectral library allows reproducible quantification for thousands of peptides per SWATH-MS analysis. Proteins from Drosophila melanogaster embryo, adult flies were digested with trypsin using in-gel digestion and the peptides were fractionated by high-pH reverse phase chromatography. HRM peptides were spiked into the peptides mixture and each fraction was injected on a Sciex TripleTOF 6600 mass spectrometer fitted with microflow set-up. The resulting .wiff files were analysed using MaxQuant and Spectronaut.

INSTRUMENT(S): TripleTOF 6600

ORGANISM(S): Drosophila Melanogaster (ncbitaxon:7227)

SUBMITTER: Kathryn Susan Lilley  

PROVIDER: MSV000081075 | MassIVE | Thu May 11 05:05:00 BST 2017

SECONDARY ACCESSION(S): PXD006495

REPOSITORIES: MassIVE