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GIGANTEA interaction proteomics time course

ABSTRACT: Interaction proteomics time course over 24h every 4h, looking for novel interactors of the circadian clock and flowering time protein GIGANTEA. Plants expressing 35S:GIGANTEA:3xFlag6His were used, as well as WT plants for a background control.

INSTRUMENT(S): QExactive

ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702)

SUBMITTER: Prof Andrew Millar

PROVIDER: MSV000081217 | MassIVE | Mon Jul 03 10:36:00 BST 2017

SECONDARY ACCESSION(S): PXD006859

REPOSITORIES: MassIVE

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Time-resolved interaction proteomics of the GIGANTEA protein under diurnal cycles in Arabidopsis.

Krahmer Johanna J Goralogia Greg S GS Kubota Akane A Zardilis Argyris A Johnson Richard S RS Song Young Hun YH MacCoss Michael J MJ Le Bihan Thierry T Halliday Karen J KJ Imaizumi Takato T Millar Andrew J AJ

FEBS letters 20181228 3

The plant-specific protein GIGANTEA (GI) controls many developmental and physiological processes, mediating rhythmic post-translational regulation. GI physically binds several proteins implicated in the circadian clock, photoperiodic flowering, and abiotic stress responses. To understand GI's multifaceted function, we aimed to comprehensively and quantitatively identify potential interactors of GI in a time-specific manner, using proteomics on Arabidopsis plants expressing epitope-tagged GI. We ...[more]

PMID: 30536871

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