Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Quantitative proteomics for dcas9 captured proteins

ABSTRACT: Quantitative proteomics analysis for dcas9 captured locus specific binding proteins with K562 cell line. The locus includes "HBB, HBG, HBD and the enhancer regions" .

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Feng Zhou

PROVIDER: MSV000081339 | MassIVE | Mon Jul 17 08:24:00 BST 2017

SECONDARY ACCESSION(S): PXD007067

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

micro-4C for investigating the interaction among HS2 core element and all other regions in β-globin locus in K562 and HL-60 cells

Project description:GATA1-binding sites constituted essential functional elements within the HS2 enhancer, enabling transcription factor recruitment, enhancer activation, and long-range chromatin interactions at the β-globin locus. In K562 cells, a human erythroleukemia line that expresses fetal and embryonic globin genes and possesses an active β-globin LCR, GATA-1 occupancy at HS2 was demonstrated by previous study. We performed micro-4C to investigate the interactions among HS2 core element and all other regions in β-globin locus in K562 and HL-60 myeloid leukemia cells (as a negative control). Erythroid-specific DNA interactions can be readily detected

2026-04-25 | E-MTAB-16830 | biostudies-arrayexpress

K562 cell line LC-MSMS,Quantitative measurement of hydrophobicity dependent LLPS property of chromatin condensate

Project description:K562 cell line treated with 2,5-HD and proteins were captured via cross-linking. We quantified chromatin structure changes and chromatin binding proteins in K562 cell before and after 2,5-hexanediol treatment by Hi-C and Hi-MS, respectively.

2021-07-26 | PXD027565 | Pride

K562 cell lime LC-MSMS,Quantitative measurement of hydrophobicity dependent LLPS property of chromatin condensate

Project description:K562 cell line treated with 1,6-HD and proteins were captured via cross-linking. We quantified chromatin structure changes and chromatin binding proteins in K562 cell before and after 1,6-hexanediol treatment by Hi-C and Hi-MS, respectively.

2021-07-26 | PXD021434 | Pride

RNAs associated with affinity captured LINE-1 ribonucleoproteins

Project description:RNAs associated with affinity captured LINE-1 ribonucleoproteins

| PRJNA423015 | ENA

CDKN2A-specific probe captured RNA-sequencing (RNACap-Seq) (HEK293T MCF7)

Project description:The CDKN2A/B locus at 9p21.3 contains crucial tumor suppressors (P16, P14, and P15) and oncogenic lncRNA ANRIL genes. This locus is most frequently inactivated in cancer genomes by deletion and DNA methylation. However, the mechanisms coordinately regulating their expression level are far from clear. In the present study, a novel lncRNA, P14AS, was characterized in the antisense strand of the fragment near CDKN2A in human cell lines using CDKN2A-specific probe captured RNA-sequencing (RNACap-Seq).

2019-03-13 | GSE128205 | GEO

CDK6 antibody-captured chimeric band in Jurkat cell line

Project description:CDK6 antibody-captured chimeric band in Jurkat cell line

2019-05-19 | MSV000083822 | MassIVE

Multiplexed engineering and analysis of endogenous enhancer activity in single cells: Human/mouse mixing Drop-Seq experiments for K562 and MEFs

Project description:Transcriptional enhancers orchestrate cell-type specific gene expression programs critical to eukaryotic development, physiology, and disease. However, despite the large number of enhancers now identified, only a small number have been functionally assessed. Here, we develop MOsaic Single-cell Analysis by Indexed CRISPR Sequencing (Mosaic-seq), a method that measures one direct phenotype of enhancer repression: change of the transcriptome, at the single cell level. Using dCas9-KRAB to suppress enhancer function, we first implement a multiplexed system to allow the simultaneous measurement of the transcriptome and detection of sgRNAs by single cell RNA sequencing. We validate this approach by targeting the HS2 enhancer in the well-studied beta-globin locus. Next, through computational simulation, we demonstrate strategies to robustly detect changes in gene expression in these single cell measurements. Finally, we use Mosaic-seq to target 71 hypersensitive regions belonging to 15 super-enhancers in K562 cells by utilizing a lentiviral library containing 241 unique-barcoded sgRNAs. Our results demonstrate that Mosaic-seq is a reliable approach to study enhancer function in single cells in a high-throughput manner.

2017-04-13 | GSE81848 | GEO

Multiplexed engineering and analysis of endogenous enhancer activity in single cells: Mosaic-Seq of beta-globin locus (separate infection)

2017-04-13 | GSE81748 | GEO

Multiplexed engineering and analysis of endogenous enhancer activity in single cells: Mosaic-Seq of beta-globin locus (pooled infection)

2017-04-13 | GSE81747 | GEO

Multiplexed engineering and analysis of endogenous enhancer activity in single cells: Bulk RNA-Seq validation

2017-04-13 | GSE81412 | GEO