Project description:Different versions (full length and various mutants) of SAP25 tagged with an N-terminal Halo affinity tag were expressed in 293T cells. The resulting lysates were used to capture SAP25 associated proteins. Purified protein complexes were identified by AP-MS.
Project description:HEK293T proteome (single shot) with knock-in or not for RPS3-Halo and Knock out or not for NUFIP1. Cells were treated or not with Torin1 or deprived of amino acids.