Proteomics

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Youn_et_al_RNAbodies_SAINT3105_Set3

ABSTRACT: This set of submissions contains the mass spectrometry files for the manuscript by Ji-Young Youn et al. that describes the high-density proximity mapping of RNA bodies. BioID experiments were performed from Flp-In T-REx HEK293 cells and MS files were acquired on Orbitrap Elite and Orbitrap Velos. Different MassIVE submissions corresponding to SAINT 3105 (see list below) have been made and this is set 3.

INSTRUMENT(S): LTQ Velos, LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Anne-Claude Gingras  

PROVIDER: MSV000081413 | MassIVE | Fri Aug 04 14:41:00 BST 2017

SECONDARY ACCESSION(S): PXD007201

REPOSITORIES: MassIVE

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Publications

mRNA processing, transport, translation, and ultimately degradation involve a series of dedicated protein complexes that often assemble into large membraneless structures such as stress granules (SGs) and processing bodies (PBs). Here, systematic in vivo proximity-dependent biotinylation (BioID) analysis of 119 human proteins associated with different aspects of mRNA biology uncovers 7424 unique proximity interactions with 1,792 proteins. Classical bait-prey analysis reveals connections of hundr  ...[more]

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