Shenmai iTRAQ proteome
Ontology highlight
ABSTRACT:
INSTRUMENT(S): TOF/TOF 5800
ORGANISM(S): Rattus (ncbitaxon:10114)
SUBMITTER: 
Yi Wang
PROVIDER: MSV000081517 | MassIVE |
REPOSITORIES: MassIVE
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Myocardial tissue of Shenmai-treated mice with left coronary artery ligation were harvest after 7 days treatment. Tissue samples were homogenized with lysis buffer by needle sonication. The solubilized samples were centrifuged at 16,000 � g for 10 min at 4 �C and the total protein in the supernatant was quantified using BCA assay. The relative quantification was calculated based on the ratio of the reporter ions intensities at m/z 117.1, 118.1, 119.1, and 121.1 corresponding to the myocardial ischemia condition as well as treated with RG, OP, and SM, over the reporter ion intensity at m/z 116.1 corresponding to sham-operated condition.
The peak lists were submitted for database sequence searches using ProteinPilot software (version 3.0, Applied Biosystems). The following parameters were set in the searching: trypsin as enzyme, fixed modification of carbamidomethyl labeled cysteine, iTRAQ as sample type, no special factors, biological modification, thorough identification search. Other parameters, such as tryptic cleavage specificity, precursor ion mass accuracy, and fragment ion mass accuracy were built-in functions of ProteinPilot software, and the Paragon method was adopted.
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