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Proteome Plasticity in Drosophila Hybrids

ABSTRACT: This submission contains 3 different datasets: - series A: D. melanogaster female ? D. simulans male embryos vs internal standard of pooled D. simulans and D. melanogaster (1:1) embryos - series B: D. simulans female ? D. melanogaster male embryos vs internal standard of pooled D. simulans and D. melanogaster (1:1) embryos - series E: D. melanogaster female ? D. simulans male adult heads vs internal standard of pooled D. simulans and D. melanogaster (1:1) adult heads Each dataset is in triplicates. Quantitation was performed with isobaric isotopologues labelling.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Drosophila Melanogaster (ncbitaxon:7227) Drosophila Simulans (ncbitaxon:7240)

SUBMITTER: John R. Yates III

PROVIDER: MSV000081535 | MassIVE |

SECONDARY ACCESSION(S): PXD007746

REPOSITORIES: MassIVE

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Increased proteomic complexity in Drosophila hybrids during development.

Bamberger Casimir C Martínez-Bartolomé Salvador S Montgomery Miranda M Lavallée-Adam Mathieu M Yates John R JR

Science advances 20180207 2

Cellular proteomes are thought to be optimized for function, leaving no room for proteome plasticity and, thus, evolution. However, hybrid animals that result from a viable cross of two different species harbor hybrid proteomes of unknown complexity. We charted the hybrid proteome of a viable cross between Drosophila melanogaster females and Drosophila simulans males with bottom-up proteomics. Developing hybrids harbored 20% novel proteins in addition to proteins that were also pre ...[more]

PMID: 29441361

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