Proteomics

Dataset Information

60

HeLa proteome of 12,250 protein-coding genes


ABSTRACT: We developed an optimized multi-shot proteomics workflow based on high-resolution offline high pH reversed-phase peptide separation of high peptide loads collecting many fractions that were in turn analyzed by short online chromatographic separations and fast peptide sequencing using orbitrap tandem mass spectrometry.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo sapiens  

SUBMITTER: Jesper V. Olsen  

PROVIDER: MSV000081563 | MassIVE | Mon Sep 25 20:36:00 BST 2017

SECONDARY ACCESSION(S): PXD004452

REPOSITORIES: MassIVE

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This study investigates the challenge of comprehensively cataloging the complete human proteome from a single-cell type using mass spectrometry (MS)-based shotgun proteomics. We modify a classical two-dimensional high-resolution reversed-phase peptide fractionation scheme and optimize a protocol that provides sufficient peak capacity to saturate the sequencing speed of modern MS instruments. This strategy enables the deepest proteome of a human single-cell type to date, with the HeLa proteome se  ...[more]

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