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Proteomic analysis of trachea and midgut from larvae of Drosophila melanogaster

ABSTRACT: Total protein was isolated from the trachea and midgut of D. melanogaster 3rd instar larvae and analysed by shotgun proteomics in order to identify the putative carbohydrate-active enzymes.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Drosophila Melanogaster (ncbitaxon:7227)

SUBMITTER: Simon J. McQueen-Mason

PROVIDER: MSV000081913 | MassIVE | Thu Jan 11 05:41:00 GMT 2018

SECONDARY ACCESSION(S): PXD008658

REPOSITORIES: MassIVE

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An ancient family of lytic polysaccharide monooxygenases with roles in arthropod development and biomass digestion.

Sabbadin Federico F Hemsworth Glyn R GR Ciano Luisa L Henrissat Bernard B Dupree Paul P Tryfona Theodora T Marques Rita D S RDS Sweeney Sean T ST Besser Katrin K Elias Luisa L Pesante Giovanna G Li Yi Y Dowle Adam A AA Bates Rachel R Gomez Leonardo D LD Simister Rachael R Davies Gideon J GJ Walton Paul H PH Bruce Neil C NC McQueen-Mason Simon J SJ

Nature communications 20180222 1

Thermobia domestica belongs to an ancient group of insects and has a remarkable ability to digest crystalline cellulose without microbial assistance. By investigating the digestive proteome of Thermobia, we have identified over 20 members of an uncharacterized family of lytic polysaccharide monooxygenases (LPMOs). We show that this LPMO family spans across several clades of the Tree of Life, is of ancient origin, and was recruited by early arthropods with possible roles in remodeling endogenous ...[more]

PMID: 29472725

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