Proteomics

Dataset Information

20170127_Zhe_MCF7_TDCID_200min


ABSTRACT: This top-down MS data set was generated from MCF-7 cells. MCF-7 cells were obtained from American Tissue Culture Collection (ATCC) and maintained in minimum essential media with 5% charcoal-dextran treated fetal calf serum. In the MS experiment, soluble MCF-7 intact proteins were separated using the bead-beating based cell lysis approach followed by a lter-based desalting step. A liquid chromatograph system with a home-made long column was directly coupled with an LTQ Orbitrap Velos Pro mass spectrometer with a customized ion source. A total of 5 309 CID MS/MS spectra were collected at a 60 000 resolution.

INSTRUMENT(S): LTQ Orbitrap Velos Pro mass spectrometer

ORGANISM(S): Mcf7

SUBMITTER: Xiaowen Liu  

PROVIDER: MSV000082061 | MassIVE | Mon Feb 12 19:29:00 GMT 2018

REPOSITORIES: MassIVE