Chinese Hamster Ovary cell proteins co-purified with recombinant HIV-1 envelopes, part 3

ABSTRACT: Chinese Hamster Ovary (CHO) cells are frequently used to produce recombinant HIV envelope protein gp120. In this study, we characterized the CHO proteins that become co-purified with gp120 when lectin affinity chromatography is used. Many of co-purified CHO proteins identified were extracellular matrix components. We verified a method for separating gp120 from these CHO proteins with anion exchange chromatography, and assessed the biochemical activity of gp120 preparations with and without co-purified CHO proteins.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Cricetulus Griseus (ncbitaxon:10029)

SUBMITTER: Shiu-Lok Hu  

PROVIDER: MSV000082065 | MassIVE | Wed Feb 14 10:11:00 GMT 2018

SECONDARY ACCESSION(S): PXD006062

REPOSITORIES: MassIVE