The nucleosomal acidic patch relieves auto-inhibition by the ISWI remodeler SNF2h

ABSTRACT: Domain level interactions between the ISWI remodeler SNF2h and reconstituted mononucleosomes were probed by EDC crosslinking under different biochemical conditions: ADP, ADP-BeFx, ADP-BeFx with LANA peptide added (which binds at the acidic patch). A semi quantitative approach was used to monitor domain-domain interactions enriched during the remodeling cycle.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Xenopus Laevis (ncbitaxon:8355) Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Geeta Narlikar  

PROVIDER: MSV000082136 | MassIVE | Wed Mar 07 16:27:00 GMT 2018

REPOSITORIES: MassIVE