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The nucleosomal acidic patch relieves auto-inhibition by the ISWI remodeler SNF2h

ABSTRACT: Domain level interactions between the ISWI remodeler SNF2h and reconstituted mononucleosomes were probed by EDC crosslinking under different biochemical conditions: ADP, ADP-BeFx, ADP-BeFx with LANA peptide added (which binds at the acidic patch). A semi quantitative approach was used to monitor domain-domain interactions enriched during the remodeling cycle.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Xenopus Laevis (ncbitaxon:8355) Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Geeta Narlikar

PROVIDER: MSV000082136 | MassIVE | Wed Mar 07 16:27:00 GMT 2018

REPOSITORIES: MassIVE

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The nucleosomal acidic patch relieves auto-inhibition by the ISWI remodeler SNF2h.

Gamarra Nathan N Johnson Stephanie L SL Trnka Michael J MJ Burlingame Alma L AL Narlikar Geeta J GJ

eLife 20180417

ISWI family chromatin remodeling motors use sophisticated autoinhibition mechanisms to control nucleosome sliding. Yet how the different autoinhibitory domains are regulated is not well understood. Here we show that an acidic patch formed by histones H2A and H2B of the nucleosome relieves the autoinhibition imposed by the AutoN and the NegC regions of the human ISWI remodeler SNF2h. Further, by single molecule FRET we show that the acidic patch helps control the distance travelled per translocat ...[more]

PMID: 29664398

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