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LKB1 loss links serine metabolism to DNA methylation and tumorigenesis

ABSTRACT: Proteome maps were generated for pancreatic ductal epithelial cells from LSL KRASG12D/+ and LSL KRASG12D/+;LKB1l/l mice. The cells were isolated from the mice, and genetic lesions were induced in vitro by infection with Ade-Cre. Proteome maps were done with multiplexed proteomics using TMT10plex technology. Mass spectrometry was done on an Orbitrap Fusion using the SPS-MS3 method. Samples were TMT labeled as follows: K1-1 (126), K1-2 (129n), K2-1 (127n), K2-2 (129c), KL1-1 (127c), KL1-2 (130n), KL2-1 (128n), KL2-2 (130c).

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Nabeel Bardeesy

PROVIDER: MSV000082186 | MassIVE | Tue Mar 20 08:55:00 GMT 2018

REPOSITORIES: MassIVE

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LKB1 loss links serine metabolism to DNA methylation and tumorigenesis.

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