Escherichia coli K-12 CIDETD

ABSTRACT: Protein extract of Escherichia coli K-12 MG1655 was separated by RPLC on a Waters NanoAquity system with a custom packed C5 column and analyzed by an LTQ Orbitrap Velos mass spectrometry. Parent spectra were collected at a 60000 resolution and the top 4 ions were selected for LC-MS/MS analysis in which the resolution was 60000 and the alternating fragmentation mode was used. In total, 2027 collision-induced dissociation (CID) and 2027 electron-transfer dissociation (ETD) top-down MS/MS spectra were collected.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Escherichia Coli K-12 (ncbitaxon:83333)

SUBMITTER: Xiaowen Liu   Si Wu  

PROVIDER: MSV000082237 | MassIVE | Wed Apr 04 07:54:00 BST 2018

REPOSITORIES: MassIVE