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Mouse muscle mitochondrial proteome changes in high fat diet vs. low fat diet

ABSTRACT: We investigated remodeling of the mitochondrial proteome to determine mechanisms of changes to lipid oxidation following high-fat feeding. C57BL/6J mice consumed either a high-fat diet (HFD, 60% fat) or low fat diet (LFD, 10% fat) for 12 weeks. Mice were fasted 4 hours then anaesthetized by sodium pentobarbital for tissue collection. A mitochondrial-enriched fraction was prepared from gastrocnemius muscles and underwent proteomic analysis by high-resolution mass spectrometry.

INSTRUMENT(S): QExactive

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Matthew Robinson

PROVIDER: MSV000082272 | MassIVE | Thu Apr 12 10:08:00 BST 2018

REPOSITORIES: MassIVE

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