HLA-DO Modulates the Diversity of the MHC-II Self-peptidome

ABSTRACT: HLA-DR1 bound peptides of five biological replicates of LG2-WT(012916_WT1,022216_WT1, 02080316MHCIIWT1, 080316MHCIIWT2, 080316MHCIIWT3) and DO-KO Clone 1 LG-2 cells (012916_KO1, 022216_DO1, 081216MHCIIKO1, 080416MHCIIKO2, 080416MHCIIKO3) were processed in parallel. Each biological replicate has 3 technical replicates. In manuscript, these biological replicates are labeled as WTa, WTb, WTc,WTd, WTe,DOa, DOb,DOc, DOd, DOe respectively. HLA-DR1 bound peptides from three biological replicates of LG2-WT and DO-KO Clone 2 were processed in parallel. Each biological replicate had 3 technical replicates. H2-IAb bound peptides of mice splenic B cells from three biological replicates of WT and H2-O KO mice were processed in parallel. Each biological replicate had 3 technical replicates.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Lawrence J. Stern  

PROVIDER: MSV000082570 | MassIVE | Tue Jul 03 18:28:00 BST 2018

SECONDARY ACCESSION(S): PXD010301

REPOSITORIES: MassIVE