N Gene TurboID (Group2 +p50)

ABSTRACT: Nicotiana benthamiana plants were infiltrated with three different constructs: 1) N-TurboID-3HA 2) NTIR-TurboID-3HA or 3) Citrine-TurboID-3HA. N-TurboID is the full length N-gene fused to TurboID, NTIR-Turbo is the TIR domain of the N-gene fused to turboID, and Citrine-TurboID is the control construct. Three biological replicates for each construct were labeled with TMT10Plex reagents (Lot TB260979). N-TurboID (TMT: 126, 127N, 126C); NTIR-TurboID (TMT: 128N, 128C, 129N); citrine-TurboID (TMT: 129C, 130N, 130C). Biotinolayted proteins were enriched with streptavidin beads (equal protein input onto beads) and digested into peptides with Trypsin/LysC. An equal volume of peptides were labeled with TMT reagents.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Nicotiana Benthamiana (ncbitaxon:4100)

SUBMITTER: Justin Walley  

PROVIDER: MSV000083019 | MassIVE | Wed Oct 10 09:00:00 BST 2018

REPOSITORIES: MassIVE