The mRNA-bound proteome of Leishmania mexicana is stage-regulated with distinct transcript target pools

ABSTRACT: Leishmania species parasite infections, termed the leishmaniases, cause significant global infectious disease burden. The lifecycle of the parasite embodies three main stages that require precise coordination of gene regulation to survive drastic environmental shifts transitioning from sandfly to mammalian hosts. Constitutive transcription in these kinetoplastid parasites is overwhelmingly reliant on post-transcriptional mechanisms, yet strikingly few Leishmania trans-regulator proteins are known. Utilizing optimised crosslinking and in-depth, quantified mass spectrometry, we present a comprehensive analysis of over 1,400 mRNA binding proteins (mRBPs) and whole cell proteomes from the three main lifecycle stages.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Leishmania Mexicana (ncbitaxon:5665)

SUBMITTER: Dr. Pegine Walrad  

PROVIDER: MSV000083023 | MassIVE | Thu Oct 11 01:41:00 BST 2018

SECONDARY ACCESSION(S): PXD011340

REPOSITORIES: MassIVE