Proteomics

Dataset Information

0

Protein O-glycoproteome in Streptomyces coelicolor


ABSTRACT: The membrane O-glycoproteome in Streptomyces coelicolor J1929 was isolated and characterised by LC-ESI-MS/MS, using CID, HCD and ETD fragmentation. Streptomyces coelicolor J1929 was grown in phosphate limiting (F134) medium, and the membrane proteins were isolated after 20, 35, 43 and 60 h of growth. Glycoproteins were enriched using lectin affinity chromatography (Con A) and the enriched fractions were analysed by LC-ESI-CID-MS/MS on a Bruker maXis mass spectromter. The enriched glycoproteins isolated after 43 h of growth were further analysed by LC-MS/MS on an Orbitrap Fusion Tribrid mass spectrometer. The S. coelicolor glycopeptides in this experiment were analysed using both HCD and ETD fragmentation, and using both the ion trap (low resolution, high speed - mass accuracy < 0.5 Da) and Orbitrap (high resolution, lower speed - mass accuracy < 3 ppm) mass analysers. Four different data acquisitions were performed to include these options: MS in Orbitrap, HCD measured in ion trap (HCD_IT), MS in Orbitrap, ETD measured ion trap (ETD_IT), MS in Orbitrap, ETD measured in Orbitrap (ETD_OT), MS in Orbitrap, HCD measured in ion trap and ETD measured in Orbitrap (HCD_IT; ETD_OT).

INSTRUMENT(S): maXis, Orbitrap Fusion

ORGANISM(S): Streptomyces Coelicolor A3(2) J1929

SUBMITTER: Professor Maggie Smtih  

PROVIDER: MSV000083115 | MassIVE | Wed Nov 07 06:25:00 GMT 2018

SECONDARY ACCESSION(S): PXD011607

REPOSITORIES: MassIVE

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2014-12-19 | PXD001461 | Pride
2013-05-05 | PXD000138 | Pride
2019-06-11 | PXD012247 | Pride
2013-10-10 | PXD000508 | Pride
2024-12-05 | PXD055586 | Pride
2016-05-09 | PXD000266 | Pride
2014-04-24 | PXD000070 | Pride
2019-09-27 | PXD007058 | Pride
2014-06-09 | PXD000186 | Pride
2024-05-21 | PXD044675 | Pride