PU1 interactome in human monocyte derived dendritic cells

ABSTRACT: Human monocyte-derived DC were cultured at a density of 0.5x10E6 cells/ml in RPMI-1640 supplemented with 10% FCS, 1% Pen/Strep, 10 ng/ml GM-CSF and 10 ng/ml IL4. At day 7, they were stimulated or not with 500 ng/ml LPS. Cells were collected at day 10. Whole cell lystaes from 2x10E8 cells were used for immunoprecipiation with PU.1 (T-21 SantaCruz) antibody. IgG was used as control.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens

SUBMITTER: Laura Gutierrez  

PROVIDER: MSV000083175 | MassIVE | Fri Nov 30 05:12:00 GMT 2018

SECONDARY ACCESSION(S): PXD011903

REPOSITORIES: MassIVE