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PU1 interactome in human monocyte derived dendritic cells

ABSTRACT: Human monocyte-derived DC were cultured at a density of 0.5x10E6 cells/ml in RPMI-1640 supplemented with 10% FCS, 1% Pen/Strep, 10 ng/ml GM-CSF and 10 ng/ml IL4. At day 7, they were stimulated or not with 500 ng/ml LPS. Cells were collected at day 10. Whole cell lystaes from 2x10E8 cells were used for immunoprecipiation with PU.1 (T-21 SantaCruz) antibody. IgG was used as control.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens

SUBMITTER: Laura Gutierrez

PROVIDER: MSV000083175 | MassIVE | Fri Nov 30 05:12:00 GMT 2018

SECONDARY ACCESSION(S): PXD011903

REPOSITORIES: MassIVE

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Publications

Comparison of the PU.1 transcriptional regulome and interactome in human and mouse inflammatory dendritic cells.

Scheenstra Maaike R MR Martínez-Botía Patricia P Acebes-Huerta Andrea A Brouwer Rutger W W RWW Caballero-Sánchez Noemí N Gillemans Nynke N De Bleser Pieter P Nota Benjamin B De Cuyper Iris M IM Salunkhe Vishal V Woltman Andrea M AM van de Laar Lianne L Rijkers Erikjan E Demmers Jeroen A A JAA van IJcken Wilfred F J WFJ Philipsen Sjaak S van den Berg Timo K TK Kuijpers Taco W TW Gutiérrez Laura L

Journal of leukocyte biology 20201202

Dendritic cells (DCs) are key immune modulators and are able to mount immune responses or tolerance. DC differentiation and activation imply a plethora of molecular and cellular responses, including transcriptional changes. PU.1 is a highly expressed transcription factor in DCs and coordinates relevant aspects of DC biology. Due to their role as immune regulators, DCs pose as a promising immunotherapy tool. However, some of their functional features, such as survival, activation, or migration, a ...[more]

PMID: 33289106

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