PU.1 interactome mouse bone marrow derived dendritic cells

ABSTRACT: Mouse bone marrow derived dendritic cells were generated by culturing bone marrow cells at a density of 0.5x10E6 cells/ml in RPMI-1640 supplemented with 5% FCS, 1% Pen/Strep, 5microM 2-mercaptoethanol, 20ng/ml GM-CSF. At day 7 dendritic cells were stimulated or not with 500 ng/ml LPS, and collected at day 10. 2x10E8 cells were used to prepare whole cell extracts and to perform PU.1 immunoprecipitaion with PU.1 antibody (T-21 Santa Cruz). IgG was used as control.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Laura Gutierrez  

PROVIDER: MSV000083176 | MassIVE | Fri Nov 30 05:21:00 GMT 2018

SECONDARY ACCESSION(S): PXD011904

REPOSITORIES: MassIVE