Proteomics

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Cavia porcellus spectral library

ABSTRACT: The guinea pig (cavia porcellus) is an excellent experimental model for translation to many aspects of human physiology and disease yet there is limited experimental information regarding its proteome. In an effort to overcome this gap in our knowledge, we generated a comprehensive spectral library of the guinea pig proteome. Homogenates and tryptic peptide digests were prepared from 16 tissues (brain, colon, duodenum, adipose, kidney, large intestine, liver, lung, ovaries, pancreas, placenta, skeletal muscle, small intestine, stomach, heart, uterus) and subjected to >200 DDA runs. Analysis of >250,000 peptide-spectrum matches resulted in the construction of a library of 73594 peptides corresponding to 7667 proteins.

INSTRUMENT(S): TripleTOF 6600, Q Exactive

ORGANISM(S): Cavia Porcellus (ncbitaxon:10141)

SUBMITTER: Michael J Taggart  

PROVIDER: MSV000083199 | MassIVE | Fri Dec 07 04:25:00 GMT 2018

REPOSITORIES: MassIVE

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Publications

The Generation of a Comprehensive Spectral Library for the Analysis of the Guinea Pig Proteome by SWATH-MS.

Palmowski Pawel P   Watson Rachael R   Europe-Finner G Nicholas GN   Karolczak-Bayatti Magdalena M   Porter Andrew A   Treumann Achim A   Taggart Michael J MJ  

Proteomics 20190722 15

Advances in liquid chromatography-mass spectrometry have facilitated the incorporation of proteomic studies to many biology experimental workflows. Data-independent acquisition platforms, such as sequential window acquisition of all theoretical mass spectra (SWATH-MS), offer several advantages for label-free quantitative assessment of complex proteomes over data-dependent acquisition (DDA) approaches. However, SWATH data interpretation requires spectral libraries as a detailed reference resource  ...[more]

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