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Ubiquilin interactomes from Fly

ABSTRACT: Mutations in Ubiquilins, UBQLN2, and UBQLN4 cause familial ALS. The role of UBQLNs in proteasomal degradation is well established but their role in autophagy-lysosomal clearance is poorly defined. Here, we performed Ubqn-GFP pull down from fly samples to find Ubqn binding partners.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Drosophila <fruit Fly, Genus> (ncbitaxon:7215)

SUBMITTER: Hugo J. Bellen

PROVIDER: MSV000083259 | MassIVE | Tue Dec 18 12:05:00 GMT 2018

SECONDARY ACCESSION(S): PXD012104

REPOSITORIES: MassIVE

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Ubiquilins regulate autophagic flux through mTOR signalling and lysosomal acidification.

Şentürk Mümine M Lin Guang G Zuo Zhongyuan Z Mao Dongxue D Watson Emma E Mikos Antonios G AG Bellen Hugo J HJ

Nature cell biology 20190225 3

Although the aetiology of amyotrophic lateral sclerosis (ALS) remains poorly understood, impaired proteostasis is a common feature of different forms of ALS. Mutations in genes encoding ubiquilins, UBQLN2 and UBQLN4, cause familial ALS. The role of ubiquilins in proteasomal degradation is well established, but their role in autophagy-lysosomal clearance is poorly defined. Here, we describe a crosstalk between endoplasmic reticulum stress, mTOR signalling and autophagic flux in Drosophila and mam ...[more]

PMID: 30804504

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