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Human corneocyte cross-linked envelopes are comprised of a multitude of proteins

ABSTRACT: The cornified outer layer of epidermis (stratum corneum) is our major extra-pulmonary barrier to the environment. This layer results from a well-orchestrated program of terminal differentiation producing corneocytes filled primarily with keratin intermediate filaments tightly connected by disulfide bonding. This cytoskeleton is interconnected to an isopeptide cross-linked protein envelope at the cell periphery stabilized by transglutaminase cross-linking.The envelope serves as a scaffold for deposition of the lipid barrier containing w-OH-ceramides secreted from inside the maturing keratinocytes.That defects in the transglutaminase gene TGM1 are a major cause of autosomal recessive congenital ichthyosis testifies to the importance of proper envelope formation for barrier function.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Robert H. Rice

PROVIDER: MSV000083265 | MassIVE | Wed Dec 19 17:42:00 GMT 2018

SECONDARY ACCESSION(S): PXD012122

REPOSITORIES: MassIVE

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Human stratum corneum proteomics reveals cross-linking of a broad spectrum of proteins in cornified envelopes.

Karim Noreen N Phinney Brett S BS Salemi Michelle M Wu Pei-Wen PW Naeem Muhammad M Rice Robert H RH

Experimental dermatology 20190501 5

Defects in keratinocyte transglutaminase (TGM1), resulting in an improper protein scaffold for deposition of the lipid barrier, comprise a major source of autosomal recessive congenital ichthyosis. For that reason, the composition and formation of the cornified (cross-linked) protein envelope of the epidermis have been of considerable interest. Since the isopeptide cross-linked protein components are not individually isolable once incorporated, purified envelopes were analysed by mass spectromet ...[more]

PMID: 30916809

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