The N-cadherin interactome in primary cardiomyocytes as defined by quantitative proximity proteomics

ABSTRACT: Low levels of Cdh2-BioID2 were expressed in mouse primary neonatal cardiomyocytes using an adenoviral expression system. Infected cells were treated with biotin for 24 hours before lysis. Biotinylated proteins were precipitated from lysates using streptavidin beads. Protein samples were in-gel trypsin digested and the resulting tryptic peptides were analyzed on an LTQ Orbitrap Velos.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Adam V. Kwiatkowski  

PROVIDER: MSV000083362 | MassIVE | Thu Jan 24 18:34:00 GMT 2019

SECONDARY ACCESSION(S): PXD012496

REPOSITORIES: MassIVE