Sensitive determination of proteolytic proteoforms in limited microscale proteome samples-Development of High-efficiency Undecanal-based N Termini EnRichment (HUNTER)

ABSTRACT: Protein N-termini reveal fundamental regulatory mechanisms and their perturbation in disease. We present a robust, sensitive, scalable and automatable method called High-efficiency Undecanal-based N Termini EnRichment (HUNTER) for system-wide identification of thousands of N-termini from minute samples. Optimization and automation of the HUNTER protocol was based on HeLa cell lysates and commercially-available plasma.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Dr. Philipp Lange   Dr. Pitter F. Huesgen  

PROVIDER: MSV000083501 | MassIVE | Thu Feb 28 17:23:00 GMT 2019

SECONDARY ACCESSION(S): PXD012915

REPOSITORIES: MassIVE