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Pancreatic beta cell proteome with increased proliferation in NOD mice

ABSTRACT: We investigated the pancreatic beta cell proteome changes in NOD-LIRKO vesus NOD where beta cell mass was enhanced early in life. Proteomics data is derived from FACS-sorted beta cells at 8 weeks and 12 weeks.

INSTRUMENT(S): LTQ Orbitrap Velos, Q Exactive

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Wei-Jun Qian

PROVIDER: MSV000083576 | MassIVE | Thu Mar 14 15:31:00 GMT 2019

SECONDARY ACCESSION(S): PXD013100

REPOSITORIES: MassIVE

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Increased β-cell proliferation before immune cell invasion prevents progression of type 1 diabetes.

Dirice Ercument E Kahraman Sevim S De Jesus Dario F DF El Ouaamari Abdelfattah A Basile Giorgio G Baker Rocky L RL Yigit Burcu B Piehowski Paul D PD Kim Mi-Jeong MJ Dwyer Alexander J AJ Ng Raymond W S RWS Schuster Cornelia C Vethe Heidrun H Martinov Tijana T Ishikawa Yuki Y Teo Adrian Kee Keong AKK Smith Richard D RD Hu Jiang J Haskins Kathryn K Serwold Thomas T Qian Wei-Jun WJ Fife Brian T BT Kissler Stephan S Kulkarni Rohit N RN

Nature metabolism 20190506 5

Type 1 diabetes (T1D) is characterized by pancreatic islet infiltration by autoreactive immune cells and a near-total loss of β-cells<sup>1</sup>. Restoration of insulin-producing β-cells coupled with immunomodulation to suppress the autoimmune attack has emerged as a potential approach to counter T1D<sup>2-4</sup>. Here we report that enhancing β-cell mass early in life, in two models of female NOD mice, results in immunomodulation of T-cells, reduced islet infiltration and lower β-cell apoptos ...[more]

PMID: 31423480

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