Proteomics

Dataset Information

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Ovine embryo - descriptive analysis


ABSTRACT: 1234567890 1234567890 1234567890 1234567890 1234567890 1234567890 1234567890

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Ovis Aries (ncbitaxon:9940)

SUBMITTER: Arlindo Moura  

PROVIDER: MSV000083725 | MassIVE | Thu Apr 25 11:55:00 BST 2019

SECONDARY ACCESSION(S): PXD013638

REPOSITORIES: MassIVE

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Publications


The present study was conducted to decipher the proteome of in vivo-produced pre-implantation ovine embryos. Ten locally adapted Morana Nova ewes received hormonal treatment and were inseminated 12 hr after ovulation. Six days later, 54 embryos (morula and blastocyst developmental state) were recovered from eight ewes and pooled to obtain sufficient protein for proteomic analysis. Extracted embryo proteins were analysed by LC-MS/MS, followed by identification based on four database searches (PEA  ...[more]

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