Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Arabidopsis thaliana mutant sr45-1 inflorescence protein TMT-6

ABSTRACT: Arabidopsis thaliana mutant sr45-1 has an altered flower shape. sr45 is a splicing regulator. In this study, we examined the proteins from inflorescence of sr45-1 mutant plants and wild-type. Wild type TMT labels: 126, 128, 130. sr45-1 TMT labels: 127, 129, 131.

INSTRUMENT(S): Lumos tribrid

ORGANISM(S): Arabidopsis Thaliana

SUBMITTER: Bret Cooper

PROVIDER: MSV000083728 | MassIVE | Fri Apr 26 09:18:00 BST 2019

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Similar Datasets

Sexual Dimorphism in the Extracellular and Pericellular Matrix of Articular Cartilage

Project description:Male and female collagen-enriched fraction of cartilage from bovine knees. Full thickness cartilage plugs without subchondral bone were digested with chondroitinase for 24 hours to remove proteoglycans. Cartilage was further decellularized with SDS. LUM1_811145: channels 126, 127, 128 female, channels 129, 130, 131 male LUM1_833734: channels 126, 127, 128 female, channels 129, 130, 131 male

| MSV000092997 | MassIVE

Low abundance of the matrix arm of complex I in the mitochondria predicts longevity

Project description:The mouse liver mitochondrial proteome was analysed in four different mouse groups (allocation of the samples to Exp1 and Exp2 in brackets): AL (ad libitum fed) C57Bl6 (Exp1 129, Exp1 130, Exp2 130), DR (dietary restriction) C57Bl6 (Exp1 126, Exp1 127, Exp1 128), AL ICRFa (Exp2 128, Exp2 129) and old ICRFa (Exp2 126, Exp2 127). To achieve quantitative standardisation between Exp1 and Exp2, all 10 samples were pooled and one portion of the pool was anlaysed in each Exp (Exp1 131 and Exp2 131). Following tryptic digest and TMT 6-plex labelling, samples were separated by OffGel electrophoresis and all fractions were subsequently analysed by LCMSMS on an Orbitrap LTQ XL. One survey scan (res. 30,000) was followed by a high energy HCD scan (res. 6000) and a low energy CID scan in the LTQ. HCD data were used for the quantitation and CID data for the identification of peptides. Data were searched using Mascot (v. 2.2) through the Proteome Discoverer interface. Peptide raw quantitations were extracted as text files and further processed using dpeaqms (http://r-forge.r-project.org/projects/dpeaqms/) to obtain probability values for the differences in protein amounts for specific proteins between the different animal groups.

2014-03-18 | PXD000272 | Pride

Bean leaves treated with benzothiadiazole (BTH)

Project description:Bean leaves treated with BTH or water. 126 = control R1, 127 = BTH R1, 128 = control R2, 129 = BTH R2, 130 = control R3, 131 = BTH R3

2019-07-31 | MSV000084162 | MassIVE

Structural Organization of the Retriever-CCC endosomal recycling complex

Project description:PM proteomics by TMT6plex, comparing WT to EV. 126: EV, rep1 127: EV, rep2 128: EV, rep3 129: WT, rep1 130: WT, rep2 131: WT, rep3

2023-06-05 | MSV000092103 | MassIVE

Autophagy collaborates with apoptosis pathways to control oligodendrocyte number

Project description:The samples are in vitro cultured oligodendrocyte precursor cells in differentiation medium for 3 days. There are 2 groups: D3HET (ATG5F/+ CNP-Cre, TMT channels 126, 127, 128) and D3KO (ATG5F/F; CNP-Cre, TMT channels 129, 130, 131), with each group comprising 3 biological replicates. Subsequently, all the samples were lysed and subjected to TMT (Tandem Mass Tag) labeling, high pH fractionation into 8 fractions (F1-F8).

2023-07-13 | MSV000092434 | MassIVE

RTNs and DP1 Knockdown in neurons

Project description:Quantitative proteomics results of RTNs-KD neurons or DP1-KD neurons (DIV5). Peptides derived from Untreated neurons (UNT) were chemically labeled with TMT-126, pSuper-neurons with TMT-127, shRNAs_RTNs-neurons with TMT-128 and shRNA_DP1-neurons with TMT-131.

2019-02-15 | PXD012264 | Pride

TMT isobaric profiling of young versus old haematopoietic stem cells

Project description:Mass spectrometry was performed with an Orbitrap Fusion Tribrid mass spectrometer (Thermo Scientific) interfaced with an UltiMate 3000 Binary RSLCnano System (Dionex). Proteome Discoverer v.1.4 (Thermo Scientific) with SEQUEST HT search engines was used for the spectra-preprocessing and HCD MS2 spectra were used for peptide identification and quantitation based on TMT reporter ions. TMT isobaric comparison of old versus young haematopoietic stem and progenitor cells. Young 1 and Young 2 are samples 126 and 128 of dataset UTH_1. Old 1 and Old 2 are samples 129 and 130 of UTH_1. Young 3 is sample 131 and Old 3 is sample 130 of dataset UTH_4.

2023-10-16 | MSV000093126 | MassIVE

Lysate samples of MEF feeder layer and naive human pluripotent stem cells cultured in microfluidics.

Project description:This is a TMT6 sample containing the following sub-samples: TAG 126: standard, used for normalization purposes, It is a mixture of different biological samples. TAG 127: Lysate of replication-incompetent MEFs cultured on VTN coating in RSeT medium. TAG 128: Lysate of naive human pluripotent stem cells cultured on a MEF feeder-layer in RSeT medium (mixed human and mouse cell population). TAG 129: Lysate of naive human pluripotent stem cells cultured on VTN coating in RSeT medium. TAG 130: Not relevant. TAG 131: standard, used for normalization purposes, It is a mixture of different biological samples. Same as sub-sample with tag 126. Detailed culture and processing methods are described in Cesare et al, under submission.

| MSV000086069 | MassIVE

Proteome expression changes upon Halofuginone treatment

Project description:NHCF-V primary cardiac fibroblasts where treated for 24 hours with either 1) 100nM Halofuginone (TMT labels 126 and 129L) 2) 300nM Halofuginone (TMT labels 127L and 129H) 3) 300 nM Halofuginone + 4 mM L-Pro (TMT labels 127H and 130L) 4) DMSO (TMT labels 128H and 131)

2017-05-16 | PXD005679 | Pride

TMT isobaric profiling of PPIA heterozygous versus knockout haematopoietic stem cells

Project description:Mass spectrometry was performed with an Orbitrap Fusion Tribrid mass spectrometer (Thermo Scientific) interfaced with an UltiMate 3000 Binary RSLCnano System (Dionex). Proteome Discoverer v.1.4 (Thermo Scientific) with SEQUEST HT search engines was used for the spectra-preprocessing and HCD MS2 spectra were used for peptide identification and quantitation based on TMT reporter ions. TMT isobaric comparison of Ppia heterozygous (het) versus knockout (KO) haematopoietic stem and progenitor cells. Het 1 is sample 126 and KO 1 is sample 131 of dataset UTH_2. Het 2 and KO 2 are samples 126 and 127 of UTH_4. Het 3 and KO 3 are samples 130 and 127 of dataset UTH_3.

2023-10-16 | MSV000093127 | MassIVE

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data