Dataset Information

Synechococcus WH8102/cyanophage S-SM1 15N incorporation

ABSTRACT: Dataset showing incorporation of extracellular nitrogen (as 15NO3) into phage proteins during infection of a marine cyanobacterium

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Synechococcus Cyanophage S-sm1 (ncbitaxon:382277) Synechococcus Sp. Wh 8102 (ncbitaxon:84588)

SUBMITTER: Jacob Waldbauer

PROVIDER: MSV000083830 | MassIVE | Mon May 20 11:36:00 BST 2019

REPOSITORIES: MassIVE

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Publications

Nitrogen sourcing during viral infection of marine cyanobacteria.

Waldbauer Jacob R JR Coleman Maureen L ML Rizzo Adriana I AI Campbell Kathryn L KL Lotus John J Zhang Lichun L

Proceedings of the National Academy of Sciences of the United States of America 20190715 31

The building blocks of a virus derived from de novo biosynthesis during infection and/or catabolism of preexisting host cell biomass, and the relative contribution of these 2 sources has important consequences for understanding viral biogeochemistry. We determined the uptake of extracellular nitrogen (N) and its biosynthetic incorporation into both virus and host proteins using an isotope-labeling proteomics approach in a model marine cyanobacterium <i>Synechococcus</i> WH8102 infected by a lyti ...[more]

PMID: 31308237

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Project description:We used microarray analysis to investigate whole genome transcriptome dynamics of the marine cyanobacterium Prochlorococcus sp. strain MED4 and the T7-like podovirus P-SSP7 over a time course during the 8 hour latent period of lytic infection prior to cell lysis. Manuscript Summary: Interactions between bacterial hosts and their viruses (phages) lead to reciprocal genome evolution through a dynamic co-evolutionary process1-5. Phage-mediated transfer of host genes – often located in genome islands – has had a major impact on microbial evolution1, 4, 6. Furthermore, phage genomes have clearly been shaped by the acquisition of genes from their hosts2, 3, 5. Here we investigate whole-genome expression of a host and phage, the marine cyanobacterium Prochlorococcus and a T7-like cyanophage during lytic infection, to gain insight into these co-evolutionary processes. While most of the phage genome was linearly transcribed over the course of infection, 4 phage-encoded bacterial metabolism genes were part of the same expression cluster, even though they are physically separated on the genome. These genes — encoding photosystem II D1 (psbA), high-light inducible protein (hli), transaldolase (talC) and ribonucleotide reductase (nrd) — are transcribed together with phage DNA replication genes and appear to make up a functional unit involved in energy and deoxynucleotide production needed for phage replication in resource-poor oceans. Also unique to this system was the upregulation of numerous genes in the host during infection. These may be host stress response genes, and/or genes induced by the phage. Many of these host genes are located in genome islands and have homologues in cyanophage genomes. We hypothesize that phage have evolved to utilize upregulated host genes, leading to their stable incorporation into phage genomes and their subsequent transfer back to hosts in genome islands. Thus activation of host genes during infection may be directing the co-evolution of gene content in both host and phage genomes. Keywords: time course, viral infection, marine cyanobacteria, podovirus, bacteriophage, stress response

Dataset Information

Synechococcus WH8102/cyanophage S-SM1 15N incorporation

Publications

Nitrogen sourcing during viral infection of marine cyanobacteria.

Similar Datasets

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