Proteomics

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Differential-Nycodenz (TMT-MS2)

ABSTRACT: Mapping the subcellular proteome using a differential fractionation and an orthogonal nycodenz fractionation combined with quantitative mass spectrometry using isobaric labeling (TMT) in MS2

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Rattus Norvegicus (ncbitaxon:10116)

SUBMITTER: Peter Lobel  

PROVIDER: MSV000083842 | MassIVE | Thu May 23 08:04:00 BST 2019

SECONDARY ACCESSION(S): PXD013993

REPOSITORIES: MassIVE

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Publications

Comparative Analysis of Quantitative Mass Spectrometric Methods for Subcellular Proteomics.

Tannous Abla A   Boonen Marielle M   Zheng Haiyan H   Zhao Caifeng C   Germain Colin J CJ   Moore Dirk F DF   Sleat David E DE   Jadot Michel M   Lobel Peter P  

Journal of proteome research 20200305 4

Knowledge of intracellular location can provide important insights into the function of proteins and their respective organelles, and there is interest in combining classical subcellular fractionation with quantitative mass spectrometry to create global cellular maps. To evaluate mass spectrometric approaches specifically for this application, we analyzed rat liver differential centrifugation and Nycodenz density gradient subcellular fractions by tandem mass tag (TMT) isobaric labeling with repo  ...[more]

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