Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

FLASHDeconv Intact protein MS1

ABSTRACT: All datasets are generated for evaluation of FLASHDecovn, thus only contain MS1. Cyto dataset, Bovine Cytochrome C (P62894 UniProt accession number), was purchased from Sigma-Aldrich. Filg dataset, filgrastim, was kindly provided by CinnaGen Co. HPLC-grade H2Oacetonitrile (ACN) and formic acid (FA) were purchased from Meck (Darmstadt, Germany). Thermo_STD dataset, Pierce Intact Protein Standard Mix, containing 6 standard proteins namely Protein G, Protein AG, IGF-I LR3, Thioredoxin, Carbonic Anhydrase II, Exo Klenow was purchased from Thermo Scientific (Bremen, Germany).

INSTRUMENT(S): Q Exactive

ORGANISM(S): Bos Taurus (ncbitaxon:9913) Streptococcus (ncbitaxon:1301) Escherichia Coli (ncbitaxon:562) Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Kyowon Jeong

PROVIDER: MSV000084001 | MassIVE | Fri Jun 21 01:15:00 BST 2019

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Datasets for FLASHQuant: a software tool for label-free quantification in top-down proteomics

Project description:Three datasets with different complexity: ProteinMix, SpikeIn, and ProteomeMix. ProteinMix is the least complex dataset consisting of LC-MS runs with a six-protein mixture (Pierce Intact Protein Standard Mix, A35526, Thermo Fisher). The six proteins are namely Protein G, Protein AG, IGF-I LR3, Thioredoxin, Carbonic Anhydrase II, and Exo Klenow, but due to the MS settings, two large proteins (Protein AG and Exo Klenow) were undetected. To generate the SpikeIn dataset, ProteinMix was spiked into an E. coli lysate in different amounts of ProteinMix (relatively diluted 1, 1/2, 1/3, 1/5, 1/7, and 1/10 with 20 ng/microlitre as 1), each of which was subject to an LC-MS run. The ProteomeMix dataset contains varying the proportion of proteins from the Human Caco-2 cell lysate (1/5, 1/2, 1, 2, and 5) with a constant concentration for E. coli lysate. The ProteinMix and SpikeIn datasets contain triplicates, and the ProteomeMix dataset includes replicates.

2023-05-12 | MSV000091923 | MassIVE

Global transcriptomic changes following elevated carbon dioxide (5%) in the wild-type (WT) and mutant (M2; RNAi-knockdown line of carbonic anhydrase (CA2)) Nannochloropsis oceanica IMET1: Illumina sequencing

Project description:In Nannochloropsis oceanica IMET1, transcript knockdown of a cytosolic carbonic anhydrase (CA2; g2018) specifically inhibited by HC resulted in ~45%, ~30% and ~40% elevation of photosynthetic oxygen evolution rate, growth rate and biomass accumulation rate under high CO2 (5% ), respectively. This CA2-knockdown mutant is demonated as M2. To probe mechanistic links underlying the mutant (M2; RNAi-knockdown line of carbonic anhydrase (CA2)) phenotypes, temporal transcriptomic profiles are compared between RNAi-knockdown line of carbonic anhydrase (CA2) and WT, at 12 h and 24h under high CO2 (5%).

2020-10-28 | GSE115445 | GEO

Differentially expressed gene profiles in CA 9-transfected C33-A, human cervix carcinoma cell line

Project description:Carbonic anhydrase IX (CA 9) is a transmembrane isoform of carbonic anhydrase (CA) that contributes to an acidification of tumor microenvironment. The expression of CA 9 in cervical tumors was shown to be strongly involved in high incidence of metastasis and poor prognosis. To search for the key regulators of metastasis related to ectopic expression of CA 9, we investigated differentially expressed gene profiles in CA 9- transfected cervix carcinoma cell line C33-A (CF) compared with mock-transfected (CP) cell line, using Affymetrix Human Genome U133 Plus 2.0 Array.

2010-03-02 | GSE20569 | GEO

carbonic anhydrase (1 or 2) simple or double mutants-Comparative transcriptome analysis between carbonic anhydrase mutants and wild type Arabidopsis

Project description:cea05-01_carbonic-anhydrase - carbonic anhydrase (1 or 2) simple or double mutants - Identification of genes differentially expressed in leaves of single CA1 and CA2 T-DNA insertional mutants and in the corresponding double mutant vs wild type - CA1 (At3g01500) and CA2 (At5g14740) T-DNA insertional mutant lines, the double (CA1+CA2) mutant and wild type Arabidopsis seeds were sown in soil in a phytotron. Leaves were harvested 40 days later for RNA extraction

2012-03-25 | GSE36625 | GEO

Effect of CA3 overexpression on signalling pathways in different cell lines

Project description:Carbonic Anhydrase 3 (CA3) was an understudied member of the carbonic anhydrase family. It is highly expressed in muscle tissue and contains two surface-exposed cysteine residues, which can undergo glutathionylation. The redox modification protects cells under oxidative stress. Recent studies have shown that CA3 interacts with Bcl-2–Associated Athanogene 3 (BAG3) and squalene epoxidase (SQLE), implicating CA3 in the pathogenesis of myasthenia gravis and non-alcoholic fatty liver disease. CA3 overexpression has also been reported to reduce hypoxia-induced apoptosis in cardiomyocytes. In this study, we performed RNA-seq profiling on HEK293T, MDA-MB-231, and SVCT cell lines following CA3 overexpression. HEK293T cells were analyzed only under normoxic conditions, whereas MDA-MB-231 and SVCT cells were cultured under both normoxia and hypoxia.

2025-11-29 | GSE311117 | GEO

Differentially expressed gene profiles in CA 9-transfected C33-A, human cervix carcinoma cell line

2010-03-06 | E-GEOD-20569 | biostudies-arrayexpress

Carbonic anhydrase

Project description:Carbonic anhydrase gene sequence from Cytobacillus oceanisediminis SBA2

| PRJEB98652 | ENA

carbonic anhydrase (1 or 2) simple or double mutants-Comparative transcriptome analysis between carbonic anhydrase mutants and wild type Arabidopsis

2012-03-25 | E-GEOD-36625 | biostudies-arrayexpress

Arabidopsis thaliana

Project description:carbonic anhydrase (1 or 2) simple or double mutants-Comparative transcriptome analysis between carbonic anhydrase mutants and wild type Arabidopsis

| PRJNA156683 | ENA

RNA-Seq analysis of Oryza sativa, ssp. japonica, cv. Kitaake leaves from plants lacking carbonic anhydrase in the chloroplast

Project description:The aim of this experiment was to assess the impact on the transcriptome of relocalisaiton of carbonic anhydrase activity from the chloroplast to the cytosol in Oryza sativa, ssp. japonica, cv. Kitaake. Four genome edited lines and one wil-type control line were analysed.

2024-06-10 | E-MTAB-14111 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data