Dataset Information

MassIVE Dataset Submission - Alnus species

ABSTRACT: LC-MS/MS data used in a work titled "Assessing Specialized Metabolite Diversity of Alnus species by Digitized LC_MS/MS Data Analysis Workflow". Negative ion mode DDA data from 15 extracts of 4 Alnus species (barks, twigs, leaves, and fruits)

INSTRUMENT(S): Xevo G2 Q-Tof

ORGANISM(S): Alnus Hirsuta Var. Sibirica (ncbitaxon:253220) Alnus Firma (ncbitaxon:109059) Alnus Japonica (ncbitaxon:109063) Alnus Hirsuta (ncbitaxon:109061)

SUBMITTER: Kyo Bin Kang

PROVIDER: MSV000084202 | MassIVE | Mon Aug 12 21:48:00 BST 2019

REPOSITORIES: MassIVE

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Publications

Assessing specialized metabolite diversity of Alnus species by a digitized LC-MS/MS data analysis workflow.

Kang Kyo Bin KB Woo Sunmin S Ernst Madeleine M van der Hooft Justin J J JJJ Nothias Louis-Félix LF da Silva Ricardo R RR Dorrestein Pieter C PC Sung Sang Hyun SH Lee Mina M

Phytochemistry 20200212

Alnus spp. (Betulaceae) have been used for treatments of hemorrhage, burn injuries, antipyretic fever, diarrhea, and alcoholism in traditional medicines. In this study, a digitized LC-MS/MS data analysis workflow was applied to provide an overview on chemical diversity of 15 Alnus extracts prepared from bark, twigs, leaves, and fruits of A. japonica, A. firma, A. hirsuta, and A. hirsuta var. sibirica. Most of the MS/MS spectra could be putatively annotated based on library matching, in silico fr ...[more]

PMID: 32062198

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Project description:Background Curculigo latifolia is a perennial plant endogenous to Southeast Asia whose fruits contain the taste-modifying protein neoculin, which binds to sweet receptors and makes sour fruits taste sweet. Although similar to snowdrop (Galanthus nivalis) agglutinin (GNA), which contains mannose-binding sites in its sequence and 3D structure, neoculin lacks such sites and has no lectin activity. Whether the fruits of C. latifolia and other Curculigo plants contain neoculin and/or GNA family members was unclear. Results Through de novo RNA-seq assembly of the fruits of C. latifolia and the related C. capitulata and detailed analysis of the expression patterns of neoculin and neoculin-like genes in both species, we assembled 85,697 transcripts from C. latifolia and 76,775 from C. capitulata using Trinity and annotated them using public databases. We identified 70,371 unigenes in C. latifolia and 63,704 in C. capitulata. In total, 38.6% of unigenes from C. latifolia and 42.6% from C. capitulata shared high similarity between the two species. We identified ten neoculin-related transcripts in C. latifolia and 15 in C. capitulata, encoding both the basic and acidic subunits of neoculin in both plants. We aligned these 25 transcripts and generated a phylogenetic tree. Many orthologs in the two species shared high similarity, despite the low number of common genes, suggesting that these genes likely existed before the two species diverged. The relative expression levels of these genes differed considerably between the two species: the transcripts per million (TPM) values of neoculin genes were 60 times higher in C. latifolia than in C. capitulata, whereas those of GNA family members were 15,000 times lower in C. latifolia than in C. capitulata. Conclusions The genetic diversity of neoculin-related genes strongly suggests that neoculin genes underwent duplication during evolution. The marked differences in their expression profiles between C. latifolia and C. capitulata may be due to mutations in regions involved in transcriptional regulation. Comprehensive analysis of the genes expressed in the fruits of these two Curculigo species helped elucidate the origin of neoculin at the molecular level.

Dataset Information

MassIVE Dataset Submission - Alnus species

Publications

Assessing specialized metabolite diversity of Alnus species by a digitized LC-MS/MS data analysis workflow.

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