TWEAK/Fn14 signalling promotes cholangiocarcinoma niche formation and progression

ABSTRACT: Cholangiocarcinoma is a cancer of the hepatic bile ducts that is typically detected at a stage too advanced for resection. Additionally, chemotherapy is of limited efficacy, hence, novel therapeutic approaches are urgently required, including targeting of the cancer stroma. A macrophage-derived signal, tumour necrosis factor-like weak inducer of apoptosis (TWEAK), binds to cell-surface fibroblast growth factor-inducible 14 (Fn14), on cholangiocarcinoma cells to induce cytokine and chemokine expression and secretion. These TWEAK-inducible factors from cholangiocarcinoma cells can also affect macrophage polarisation. We characterised proteins secreted by four well-characterised human cholangiocarcinoma cell lines in the presence or absence of recombinant human TWEAK (100 ng/ml), to discover novel TWEAK-inducible factors that could drive pro-tumour niche formation.

INSTRUMENT(S): micrOTOF-Q II

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Prof.Stuart Forbes  

PROVIDER: MSV000084273 | MassIVE |

SECONDARY ACCESSION(S): PXD015317

REPOSITORIES: MassIVE