The anti-MRSA compound KCR inhibits protein synthesis in Staphylococcus aureus

ABSTRACT: S aureus was treated in triplicate with 3-O-alpha-L-(2",3"-di-p-coumaroyl)rhamnoside (KCR), oxacillin or vehicle and quantitative proteomic analysis was carried out using isobaric tags and mass spectrometry. 1190 proteins were identified and 552 were affected by KCR (q<0.01). Ontology analysis identified 6 distinct translation-related categories that were affected by KCR (PIANO, 10% false-discovery rate) including structural constituent of ribosome, translation, rRNA binding, tRNA binding, tRNA processing and aminoacyl-tRNA ligase activity. Median fold changes (KCR vs Control) for small and large ribosomal components were 1.46 and 1.43 respectively.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Staphylococcus Aureus (ncbitaxon:1280)

SUBMITTER: Nicholas Carruthers  

PROVIDER: MSV000084282 | MassIVE |

SECONDARY ACCESSION(S): PXD015333

REPOSITORIES: MassIVE