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University of Avignon Prunus persica after aphid infestation

ABSTRACT: The metabolic response of peach tree (Prunus persica) to green aphid (Myzus persicae) has been investigated by GC-MS profiling of the apex polar extracts. Six plants of two cultivars, GF305 (sensitive to M. persicae) and Rubira (resistant), were obtained from the germination of seeds after a 3 months stratification at 4C and grown for eight weeks in a green house before transfer in a growth chamber where the experiment took place. Three plants of each genotype were infested with 10 synchronized green aphid female adults (clones MP06). Aphids were removed after 48 hours and the plant apex (about 100 mg) were collected in liquid nitrogen and thoroughly ground with a ball mill.

INSTRUMENT(S): LECO Pegasus BT TOFMS

ORGANISM(S): Prunus Persica

SUBMITTER: raphael Lugan

PROVIDER: MSV000084377 | MassIVE | Wed Sep 25 10:23:00 BST 2019

REPOSITORIES: MassIVE

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Project description:A transcriptome analysis was applied on two peach (Prunus persica L.) cultivars with different sensitivity to low temperature regimes to identify cold-responsive genes that might be involved in tolerance to long low temperature storage. Peach fruit from ‘Morettini No2’ and ‘Royal Glory’, a sensitive and a tolerant, to chilling injury cultivars, respectively, were harvested at commercial maturity stage and allowed to ripen at room temperature (25°C) or subjected to 4 and 6-weeks of cold storage (0°C, 95% R.H.) followed by ripening at room temperature. Microarray experiments, employing the peach microarray platform (μ PEACH 1.0), were carried out by comparing harvested fruit against 4- and 6-week cold-stored fruit. The analysis identified 173 and 313 genes that were differentially expressed in ‘Morettini No2’ and ‘Royal Glory’ fruit after 4 weeks, respectively. However, the 6 weeks cold storage provoked a decrease in the total number of genes differentially expressed in both cultivars. RNA blot analysis validated the differential expression of certain genes showed in microarray data. Among these genes, two heat shock proteins (hsps), a putative β-D-xylosidase, an expansin, a dehydrin and a pathogenesis-related protein PR-4B precursor were induced during cold storage in both cultivars. The induction of hsps and the putative β-D-xylosidase appeared to be independent on the duration of postharvest treatment. On the other hand, transcript levels of lipoxygenase were quite constant during postharvest ripening, while a strong reduction or disappearance was observed after cold storage. A dehydration-induced RD22-like protein showed a reduction in the accumulation of transcripts during postharvest ripening independently on the temperature conditions. Overall, the current study shed some light on the molecular aspects of cold stress in peach fruit quality and identified some ripening and/or cold-induced genes which function need further elucidation.

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University of Avignon Prunus persica after aphid infestation

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