N terminome analysis of pediatric acute leukemia patients and matched xenografts

ABSTRACT: Proteomics reveal PTM stability after xenotransplantation in murine models. N termini enriched using HUNTER technique were analyzed by Data Independent Acquisition (DIA) mass spectrometry. High-pH fractionation was performed on a pool of samples, followed by data dependent acquisition (DDA) mass spectrometry analysis. Spectral information from DDA and DIA data were used to generate a sample-specific library for targeted analysis to identify and quantify proteins. We present a global landscape of proteolytic processing in paired patients and patient-derived xenografts, pediatric leukemic cell lines, and normal individuals.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Dr. Philipp Lange  

PROVIDER: MSV000084643 | MassIVE | Mon Dec 02 14:02:00 GMT 2019

SECONDARY ACCESSION(S): PXD016545

REPOSITORIES: MassIVE