Gender-related secretome of Plasmodium berghei sexual stages

ABSTRACT: In this study, we applied a proteomic approach to identify proteins differentially released/secreted by female and male gametocytes of the rodent Plasmodium berghei activated to form gametes. We compared secreted molecules of the wild type gametocytes with those of a transgenic line defective in male gamete maturation and egress. This enabled us to provide a comprehensive dataset of egress-related molecules and their gender specificity. Using specific antibodies, we validated eleven candidate molecules, predicted as either gender-specific or common to both male and female gametocytes. All of them localize to punctuate, vesicle-like structures that relocate to cell periphery upon activation, but only three of them localize to the gametocyte-specific secretory vesicles named osmiophilic bodies. Our results confirm that the egress process involves a tightly coordinated secretory apparatus that includes different types of vesicles and may put the basis for functional studies aimed at designing novel transmission-blocking molecules.

INSTRUMENT(S): LTQ XL

ORGANISM(S): Plasmodium Berghei Anka (ncbitaxon:5823)

SUBMITTER: FEDERICA FRATINI  

PROVIDER: MSV000084655 | MassIVE | Wed Dec 04 05:44:00 GMT 2019

SECONDARY ACCESSION(S): PXD016592

REPOSITORIES: MassIVE