Dataset Information
Halo-SPIN1/SNAP-SPINDOC SNAP-Halo-SCAP E2-DSSO-XL
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Orbitrap Fusion Lumos
ORGANISM(S): Homo Sapiens (ncbitaxon:9606)
SUBMITTER: 
Michael P. Washburn
PROVIDER: MSV000084719 | MassIVE | Wed Dec 18 07:09:00 GMT 2019
REPOSITORIES: MassIVE
Publications
Driving integrative structural modeling with serial capture affinity purification.
Proceedings of the National Academy of Sciences of the United States of America 20201130 50
Streamlined characterization of protein complexes remains a challenge for the study of protein interaction networks. Here we describe serial capture affinity purification (SCAP), in which two separate proteins are tagged with either the HaloTag or the SNAP-tag, permitting a multistep affinity enrichment of specific protein complexes. The multifunctional capabilities of this protein-tagging system also permit in vivo validation of interactions using acceptor photobleaching Förster resonance energ ...[more]
