Proteomics

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Effects of mobile phone electromagnetic radiation on rat hippocampus proteome


ABSTRACT: Worldwide, number of mobile phone users have increased from 5.57 billion in 2011 to 6.8 billion in 2019. However short and long term impacts of the electromagnetic radiations emitting of mobile phone on tissue homeostasis with particular to brain proteome composition needs further investigation. In this study, we attempted a global proteome profiling study of rat hippocampus exposed to mobile phone radiation for 20 weeks (for 3 hrs/day for 5 days/week) to identify deregulated proteins and western blot analysis for validation. As a result, we identified 358 hippocampus proteins, of which 16 showed deregulation (log2 (exposed/control), p-value<0.05). Majority of these deregulated proteins grouped to three clusters sharing similar molecular functions/pathways. A set of four proteins (Aldehyde dehydrogenase:Aldh5a1, Na+ K+ transporting ATPase:Atp1b2, plasma membrane calcium transporting ATPase:PMCA and protein S100b) presenting each functional pathways were selected as important molecules. Western blot analysis of this protein set, expect Atp1b2, in independent samples corroborated the mass spectrometry findings. Aldh5a1 involve in cellular energy metabolism, both Atp1b2 and PMCA responsible for membrane transport and protein S100b has neuroprotective role. In conclusion, we present deregulated hippocampus proteome upon mobile phone radiation which might impact healthy functioning of brain.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Rattus norvegicus  

SUBMITTER: Dr. Ranjan Kumar Nanda  

PROVIDER: MSV000084731 | MassIVE | Fri Dec 20 22:53:00 GMT 2019

SECONDARY ACCESSION(S): PXD016889

REPOSITORIES: MassIVE

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