Dataset Information

Proteomic Mapping of multifunctional complexes in triatomine Saliva

ABSTRACT: Salivary multiprotein complexes from Triatoma infestans, Triatoma dimidiata, Dipetalogaster maxima, Rhodnius prolixus, and Rhodnius neglectus were identified by Blue-Native-PAGE (BN-PAGE) coupled with liquid chromatography tandem mass spectrometry.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Triatominae (ncbitaxon:70999)

SUBMITTER: paula beatriz Santiago Jaime Martins de Santana Sebastien Charneau Carla Nunes de Araujo

PROVIDER: MSV000085118 | MassIVE | Wed Mar 18 18:56:00 GMT 2020

SECONDARY ACCESSION(S): PXD018101

REPOSITORIES: MassIVE

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Proteomic Mapping of Multifunctional Complexes Within Triatomine Saliva.

Santiago Paula Beatriz PB Charneau Sébastien S Mandacaru Samuel Coelho SC Bentes Kaio Luís da Silva KLDS Bastos Izabela Marques Dourado IMD de Sousa Marcelo Valle MV Ricart Carlos André O CAO de Araújo Carla Nunes CN Santana Jaime Martins JM

Frontiers in cellular and infection microbiology 20200902

Triatomines are hematophagous insects that transmit <i>Trypanosoma cruzi</i>, the etiological agent of Chagas disease. This neglected tropical disease represents a global health issue as it is spreading worldwide. The saliva of Triatominae contains miscellaneous proteins crucial for blood feeding acquisition, counteracting host's hemostasis while performing vasodilatory, anti-platelet and anti-coagulant activities, besides modulating inflammation and immune responses. Since a set of biological p ...[more]

PMID: 32984079

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Project description:The biology and physiology of Siberian sturgeon reproduction differ substantially from teleost fish. Seminal plasma support and protect the viability, motility and fertilizing capacity of spermatozoa by creating the optimal environment for the storage. The present study for the first time described in-depth proteomic characterization of Siberian sturgeon seminal plasma using gel based and gel-free proteomic approaches: LC-MS/MS, 2DE and 2D blue native (BN)/SDS-PAGE. LC-MS/MS allowed to identify 657 proteins, which were classified according to their functions and pathways. 2DE visualized 339 spots corresponding to 76 unique proteins (almost 60% were present in various proteoforms). For the first time we demonstrated interactions between seminal proteins; four (C1-C4) multiprotein complexes were identified after 2D BN/SDS-PAGE; composed of (C1) serotransferrin-2 (TF) and fish-egg lectin (FEL); (C2) serum albumin 2 (ALB) and retinol-binding protein 4 (RBP4); (C3) ALB and TF (C3) and (C4) apolipoprotein A-I (APOA1), riboflavin-binding protein (RfBP) and myoglobin (MB). BN-SDS-PAGE also revealed that immunoglobulin and TF formed homomultimeric (dimer, trimer, tetramer and pentamer) complexes in seminal plasma. ALB, TF, Beta-Ala-His dipeptidase, glyceraldehyde-3-phosphate dehydrogenase, IGH, APOA1, hemopexin, type-4 ice-structuring protein, FEL, RfBP and glycogen phosphorylase, liver form-like were selected as major seminal plasma proteins. The functional analysis of identified proteins indicated their involvement mainly in immune system process and response to stimulus, metabolism, vesicle-mediated transport, proteolysis, and catherin binding involved in cell-cell adhesion. Moreover, 67 proteins were associated with reproductive processes, including spermatogenesis, fertilization, acrosomal reaction and sperm motility. Comparative proteomic analysis of sturgeon seminal plasma showed common proteins with fish and human and 150 proteins specific for sturgeon which may reflect the specificity of sturgeon reproduction. To conclude, this integrative proteomic view lead to deeper insight into physiological function of seminal plasma and processes occurred in sturgeon reproductive tract.