The human diabetes-specific visceral adipose tissue proteome: insights into mechanisms of adipose tissue dysfunction in metabolic disease

ABSTRACT: Introduction: The human adipose tissue proteome associated with type 2 diabetes (DM) is not well described. An understanding of the DM-specific adipose tissue proteome would provide insight into mechanisms underlying the pathogenesis of DM. Methods: We used label-free proteomics analysis to quantify differences between visceral adipose tissue samples from obese women with and without DM. Results: 2640 protein groups were identified and 1965 were subject to statistical analysis. 23 were differently abundant between groups (q < 0.1, moderated t-test, n = 10). Proteins localized to the mitochondria or involved in mitochondrial functions including the TCA cycle and fatty acid metabolism were decreased in abundance in samples from women with DM relative to NDM samples while proteins involved in cytoskeletal function and innate inflammatory signaling pathways were increased. Conclusion: The visceral adipose tissue proteome in DM is characterized by defects in mitochondrial function, TCA metabolism, inflammation and immunity, and cytoskeletal function. Alterations in the levels of specific proteins associated with these pathways provide insight into mechanisms of adipose tissue dysfunction in the context of metabolic disease.

INSTRUMENT(S): Orbitrap Fusion ETD

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Robert W. O'Rourke  

PROVIDER: MSV000085169 | MassIVE | Fri Mar 27 07:25:00 GMT 2020

SECONDARY ACCESSION(S): PXD021147

REPOSITORIES: MassIVE