Automated coupling of nanodroplet sample preparation with liquid chromatography-mass spectrometry for high throughput single-cell proteomics

ABSTRACT: Development and evaluation of an autosampler for integrating nanoPOTS with LC-MS. Includes proteomic data from nanoPOTS-based sample preparation, including diluted peptides of Shewanella oneidensis MR-1, single cultured MCF10A cells, and single cells from three Acute Myeloid Leukemia (AML) cell lines (MOLM-14, K562, and CMK). Data was searched with MaxQuant.

INSTRUMENT(S): Orbitrap Fusion Lumos, Q Exactive Plus

ORGANISM(S): Shewanella Oneidensis Mr-1 (ncbitaxon:211586) Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Ying Zhu  

PROVIDER: MSV000085230 | MassIVE | Sun Apr 05 09:44:00 BST 2020

REPOSITORIES: MassIVE